T9026
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 | Immunofluorescence Chinese hamster embryonic fibroblasts in anaphase of mitosis were stained using Monoclonal Anti-α-Tubulin, clone no. DM1A (Cat. No. T9026) with Anti-Mouse-Alexa 488 (green) and DAPI (blue). From Thomas Durcan, Department of Biological Sciences, University of Notre Dame, Notre Dame, IN. |
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T9026
Sigma
Monoclonal Anti-α-Tubulin antibody produced in mouse
clone DM1A, ascites fluid
| Synonym: | Monoclonal Anti-α-Tubulin |
Description
| Immunogen | microtubules from chicken embryo brain. |
| Specificity | The antibody is specific for α-tubulin in immunoblotting assays and may be used for localization of α-tubulin in cultured cells or tissue sections. The antibody reacts best with chicken fibroblasts. |
Properties
| antibody form | ascites fluid |
| clone | DM1A (monoclonal) |
| mol wt | antigen mol wt ~50 kDa |
| contains | 15 mM sodium azide |
| species reactivity | yeast, human, chicken, rat, amphibian, fungi, bovine, mouse |
| application(s) | indirect immunofluorescence: 1:500 using cultured chicken fibroblasts |
| isotype | IgG1 |
| Gene Information | human ... TUBA4A(7277) |
| rat ... Tuba1a(64158) | |
| mouse ... Tuba1a(22142) | |
| shipped in | dry ice |
| storage temp. | −20°C |
Safety
| WGK Germany | 3 |
References
| reference | Sasaki, A., et al., Novel transcription-factor-like function of human matrix metalloproteinase 3 regulating the CTGF/CCN2 gene. Mol. Cell. Biol. 28, 2391-2413, (2008) Abstract |
| Russo, C., et al., Signaling through ShcA Is Required for Transforming Growth Factor and Neu/ErbB-2-Induced Breast Cancer Cell Motility and Invasion Mol. Cell. Biol. 28, 3162-3176, (2008) Abstract | |
| Huelsken, J., et al., Distinct requirements for Gab1 in Met and EGF receptor signaling in vivo Proc. Natl. Acad. Sci. U. S. A. 104, 15376-15381, (2007) Abstract | |
| Blose, S.H., et al., 10-nm filaments are induced to collapse in living cells microinjected with monoclonal and polyclonal antibodies against tubulin. J. Cell Biol. 98, 847, (1984) |
